Description
Product Characteristics: The EZ4U assay is a non-radioactive, non-toxic cell proliferation and cytotoxicity assay. The assay set-up is performed in a manner similar to the standard 3H-thymidine incorporation method. Instead of pulsing with tritiated nucleotide, 20 µl of dye solution is added to 200 µl sample. Incubation time is dependent on the metabolic capacity of the cells. Usually 2 to 5 hours of incubation at 37°C are sufficient to yield a significant increase in colour intensity which can be measured with a normal microplate-reader at 450nm. The EZ4U system is well suited for a variety of biological tests, where cell viability is of importance. It offers advantages over conventional dye or 3H incorporation assays. Due to its soluble end products it is easier and faster to perform than other non-radioactive cell viability assays. Furthermore, because the assay procedure is identical to the thymidine incorporation procedure, no changes in test protocols are necessary. An important benefit is the possibility of an ongoing cultivation after cell number determination and easy to adapt incubation times due to the non-toxic substrate. The EZ4U assay has been widely cited in >260 publications to date